Aflatoxin M1 determination in cheese by immunoaffinity column clean-up coupled to high-performance liquid chromatography Determinação de Aflatoxina M1 em queijos por HPLC com purificação por colunas de imunoafinidade
نویسندگان
چکیده
INRB-IP, Laboratório Nacional de Investigação Veterinária, Serviço de Micologia, Estrada de Benfica 701, 1549-011 Lisboa Faculdade de Medicina Veterinária Universidade Lusófona de Humanidades e Tecnologia, Campo Grande 376, 1749-024 Lisboa Escola Superior de Hotelaria e Turismo do Estoril, Avenida Condes de Barcelona, 2769-510 Estoril CIISA Faculdade de Medicina Veterinária TULisbon. Av. da Universidade Técnica, 1300-147 Lisboa RPCV (2007) 102 (563-564) 321-325 R E V I S T A P O R T U G U E S A
منابع مشابه
OCCURRENCE OF AFLATOXIN M1 IN PARMESAN CHEESE CONSUMED IN MINAS GERAIS, BRAZIL Ocorrência de aflatoxina M1 em queijo Parmesão consumido em Minas Gerais, Brasil
Aflatoxin M 1 (AFM 1 ) may occur in milk and milk products, resulting from the ingestion of aflatoxin B 1 in feedstuffs by dairy cow. A total of 88 samples of Parmesan cheese marketed in Minas Gerais state, Brazil, from March 2004 to December 2004, were analyzed for AFM 1 by high-performance liquid chromatography (HPLC) with a fluorescence detector following sample clean-up using immunoaffinity...
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During 2002 and 2003, a total of 107 samples of raw, pasteurized and ultrahigh treated temperature (UHT) milk commercialized in the cities of São Paulo and Marília (SP) were analyzed for the presence of aflatoxin M1 (AFM1). AFM1 was detected in 79 (73.8%) of milk samples, ranging from <0.02 to 0.26 μg/L.The samples were analyzed using an immunoaffinity column for cleanup and a thin layer chroma...
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Background & Aims: Aflatoxin M1 (AFM1) is a major metabolite of aflatoxin B1 detected in the milk of mammals that their foods have been contaminated with aflatoxins. Aflatoxins are a group of highly toxic metabolites produced by the common fungi Aspergillus flavus, A. parasiticus. They are among the most potent carcinogens found in foods. Considering its risk to the human health and high consum...
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A collaborative study was conducted to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatographic method for determination of aflatoxin M1 in milk at proposed European regulatory limits. The test portion of liquid milk was centrifuged, filtered, and applied to an immunoaffinity column. The column was washed with water, and aflatoxin was eluted with pure acetonitrile. ...
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BACKGROUND Aflatoxins are one of the most potent toxic substances that occur naturally. Nowadays extensive attention has been taken to their existence in food and environment, as there is the possibility of harm to humans following chronic exposure to extremely low levels via food chain. Aflatoxin M1 (AFM1) is a hepatic carcinogenic metabolite found in the milk of lactating animals fed with con...
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